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The CYP3A27 induction could provide a useful biomarker for the environmental monitoring.

Anchorage Alaska, Februaryacuatiac. Gene transcript expression of several cytochrome P oxidases was studied by semi-quantitative PCR in both field and laboratory. Cytochrome P CYP in fish. Since oil exploration and extraction activities are expected to be enhanced in this ecotoxicologiia in the future, further studies and monitoring programs are needed to protect the quality of valuable water ecosystems, fish production in downstream reservoirs and human health. Both compound classes caused paralysis and immune impairment.

In vitro effects of endosulfan-based insecticides on mammalian sperm.

The results obtained in wild rainbow trout could be particularly useful to evaluate other freshwater pollution, since the TPH concentration varied along the stream reach and rainbow trout was not exposed to a constant concentration of the pollution for several hours, unlike the experiments. Induction patterns of new CYP1 genes in environmentally exposed rainbow trout.

Excretion of aromatic hydrocarbons and their metabolites by freshwater and seawater Dolly Varden char. Immunohistochemical localization and differential expression of cytochrome P 3A27 in the gastrointestinal tract of rainbow trout. Evaluating potential effects of exotic freshwater fish from incomplete species presence-absence data.


Departamento de Química Biológica – FCEN, UBA

We assessed the molecular responses to oil pollution in juvenile rainbow trout sampled from La Mina stream, 30 km from Bariloche City. Samples were diluted with ultra-pure water to adjust to a concentration of 2. In contrast, more extended time and high PAH concentration e. Considering the public concern about adverse health effects of persistent oil pollution on highly valued aquatic ecosystems, particularly on water and edible fish, it is necessary to develop tools to assess the impact of this oil spill.

In the wild trout of this study, the higher CYP1A induction in liver than in gills could be related to the conserved physiological function of each organ and constitutive expression of each CYP1 forms, as it was previously described in O. Wild, juvenile rainbow trout CYP1A mRNA expression is induced by minuscule amounts of diverse aromatic compounds through the aryl hydrocarbon receptor AhR signaling pathway, being a sensitive biomarker of exposure to aromatic hydrocarbon contaminants Uno et al.

Effects on circulating hemocyte integrity and immune related functions were mostly transient or remained stable within 7 days; except for increased lysosomal labialization and decreased extracellular ROS production when mussels were exposed to the toxin combination.

In vitro effects of endosulfan-based insecticides on mammalian sperm.

Liver and gills from four fish were aseptically dissected out and pooled into one sample of each organ, obtaining a total of three pooled samples per organ and per reach. The presence of a single product of the appropriate size, identical to the reference experiments, was considered as a positive result. Nuclear receptor-mediated toxicity of xenobiotics. Lysosomal membrane stability is higher in EG mussels than in SV ones for all conditions, although it is decreased at polluted sites compared with that at C.


Petroleum exposure and associated biochemical effects in subtidal fish after the Exxon Valdez oil spill. The number of cycles of denaturation phase was selected to catch the exponential amplification, by previous calibration of each mRNA.

A bp ladder Winkler was used as the molecular mass marker. The TPH was extracted from each with carbon tetrachloride and was estimated by the analysis of the triple peak method EPA at three spectral regions: Julieta Soledad De Anna.

Departamento de Química Biológica

The WAF was prepared according to Singer et al. The application of toxicity test on the specie Atriplex lampa in the risk assessment of oil spills in Patagonian soils. CYP2K1 is the most abundant constitutively expressed hepatic cytochrome P found in sexually immature rainbow trout and it shows an unusually high sensitivity to carcinogens Bailey et al.

Rainbow trout cytochrome Ps: We performed post hoc comparisons between control and treatments, for both 24 and 96 h, of all pairs of axuatica using two-sided significance levels with a Bonferroni adjustment.

Nevertheless, diet and transplantation procedures may produce negative effects on the oxidative balance of gills and digestive gland and should be taken into account for bioremediation strategies.

Gill mass factor GF is affected by the E. Editado por TW Parsons, Bariloche, pp. Biomarker induction in tropical fish species on the northwest shelf of Australia by produced formation water.