About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.
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Additional wavelengths are obtainable using optical filters accessories. For more detailed information, refer to Pump P User Manual.
ÄKTApurifier – GE Healthcare Life Sciences
Calculating the delay volume Calculate the contribution from each component and tubing from the UV cell to the fraction collector outlet. Chemical stains and dust should be removed. D In certain cases, the D parameter can reduce the oscillations introduced by a PI-regulator. All three are loaded by a syringe. Click the xaxis to view the method in time, volume or column volumes.
The new correction factors will only apply while this method is used. All documentation about the run is stored here, e. Run Setup in the Putifier Editor for the method that is used.
To get help about an instruction or module: Up to 5 buffering components can be added. If you log on for the very first time, select user default and enter the password default.
ÄKTApurifier User Guide – GE Healthcare Life Sciences |
Use a pruifier restrictor. For example, to change the flow rate: If there is still air in the inlet tubing, stop and purge the pump according to Chapter 5 in the Pump P User Manual.
Find the thermodynamic pKa by taking the apparent pKa and add the value, found in the table below, for the buffer concentration at which the apparent pKa has been measured. To customize the Curves pane: Tubing Tefzel tubing, i.
If they become full and overflow, liquid might penetrate the system causing a shortcircuit. PressLevel is the maximum allowed pressure.
Layouts can be selected in Save current layout and all your saved selections mahual apply. This diameter should be used with columns that have a low maximum pressure limit and allow high flow rates.
Check the valves are operating correctly. The Curves pane shows curves during the run.
If the set column pressure limit is still exceeded with the i. Do not disassemble the optical unit while the lamp is ON. Settings in the System Control module and then Specials. Change channel plate and distribution plate.
If the demands on low band-broadening are less critical, use the tubing kit already installed. If not, purge the pump with a syringe as described in 2. Help us improve your experience by sending an error report.
For standard mode, four sizes of tube racks for tube diameters of 12, 18 and 30 mm tubes, and well microplates are available. Go to Section 4. To stop the run before it is finished, click the End button at the top. Mount the pH electrode in the flow cell. The PID parameters should only be changed by experienced users. To prevent pressure peaks when continuing, use a lower flow rate in the block after the Watch instruction than used before the Watch instructions.
The following topics are described: